BBE-Biotecnologie Biochimiche ed Enzimologia

Prof. Raffaele Porta

Room 2N-38/39; phone +39-081-2539473; raffaele.porta@unina.it ;

https://www.docenti.unina.it/raffaele.porta

https://orcid.org/0000-0003-4455-0664

Prof. Loredana Mariniello

Room 2N-37; phone +39-0812539470; loredana.mariniello@unina.it;

https://www.docenti.unina.it/loredana.mariniello

https://orcid.org/0000-0002-6800-5890

Prof. Prospero Di Pierro

Room 2N-34; phone +39-081674287;prospero.dipierro@@unina.it;

https://www.docenti.unina.it/prospero.dipierro

https://orcid.org/0000-0001-6094-3543

 

Dott.ssa C. Valeria L. Giosafatto

Room 2N-36; phone +39-0812539470;giosafat@unina.it

https://www.docenti.unina.it/giosafat

https://orcid.org/0000-0001-8762-2205

 

Other Group members:

PhD students: Asmaa Al-Asmar (XXXII ciclo); Manar Abdalrazeq ((XXXIII ciclo); Fatemeh Mirpour (XXXIV ciclo)

Group web sites: 

http://www.transglutaminase-what.org/

https://www.facebook.com/Biotecnologie-Biochimiche-ed-Enzimologia-BBE-1581088382151933/?ref=br_rs

Research:

Use of transglutaminase as biotechnological tool

Production and characterization of bioplastics

Use of transglutaminase as biotechnological tool

Transglutaminases (TGases; 2.3.2.13) are a family of enzymes able to catalyze the formation of isopeptide bonds between endoprotein gamma-carboxamide groups of glutamine residues and epsilon-amino groups of lysine residues, giving rise to linear or branched protein  homo- and heteropolymers. TGases, and in particular microbial molecular form of the enzyme (mTGase) exhibiting peculiar characteristics (resistance, not cation-dependency, wide range of optimal pH),   can be utilized as an effective tool in vitro to modify the structure and function of both peptides and proteins able to act as acyl donor and/or acceptor substrates for the enzyme. We carry out purification and immobilization studies of the enzyme and researches devoted to find out new TGase substrates either from animal and plant tissue to modify their biological properties, such as their antimicrobial activity, their allergenicity and digestibility.

Main collaboration:

Prof. Alan Mackie, School of Food Science & Nutrition, University of Leeds, UK

Prof. Mingsheng Dong, College of Food Science and Technology, Nanjing, Agricultural University, China

Prof. Neila Trifi-Farah, Faculté des Sciences de Tunis

Prof. Renata Piccoli, Dpt of Chemical Sciences, University of Naples Federico II

Selected publications:

  • A. Romano, C.V.L. Giosafatto, A. Al-Asmar, P. Masi, R. Romano & L. Mariniello. Structure and in vitro digestibility of grass pea (Lathyrus sativus L.) flour following transglutaminase treatment. European Food Research and Technology (2019), 245, 1899.

  • G. Xing, C.V.L Giosafatto, X. Rui, M, Dong, L,Mariniello. Microbial transglutaminase-mediated polymerization in the presence of lactic acid bacteria affects antigenicity of soy protein component present in bio-tofu. Journal of Functional Foods (2019), 53, 292.

  • A. Romano, C.V.L. Giosafatto, A. Al-Asmar, L. Mariniello (2018). Transglutaminase protein substrates of food interest. In  Kuddus M. (eds) Enzymes in Food Technology. Springer, Singapore.

  • C.V.L. Giosafatto, P. Di Pierro, R. Romano, P. Masi, L. Mariniello. Impact of transglutaminase on properties and in vitro digestibility of white bean (Phaseolus vulgaris L.) flour. Food Research International (2016) 88, 239.

  • A. Romano, C.V.L. Giosafatto, P. Masi, L. Mariniello. Impact of dehulling on the physico-chemical properties and in vitro protein digestion of common beans (Phaseolus vulgaris L.). Food & Function. (2015) 6, 1345.

Production and characterization of bioplastics

Bioplastics are an attractive eco-friendly alternative since they can be easily degraded by the enzymes present in different microorganisms. In particular, we produce hydrocolloid bioplastics by using unexpansive raw materials originating from agro-industrial wastes. These materials are made of natural biopolymers (polysaccharides, protein and their derivatives) with peculiar characteristics of mechanical resistance and gas permeability. To improve their functional properties, the enzyme transglutaminase of microbial origin  has been successfully exploited by our group during the last 15 years. In order to further strengthen the hydrocolloid film matrix, we also use different plasticizers, such as glycerol or polyamines, able to improve the technological features of the films. They are also reinforced with nanoparticles and/or blended with other hydrophobic polymers. Finally, the obtained bioplastics are exploited as carriers of active molecules for their possible application to improve the food preservation.

 

Main collaboration:

Prof. Giovanni Sannia, Dpt of Chemical Sciences, University of Naples Federico II

Prof Andreas Blennow, Dpt of Plant and Environmental Sciences, University of Copenhagen (Denmark)

Prof. Reynaldo Villalonga, Facultad de Ciencias Químicas, Complutense University of Madrid (Spain)

Prof. Carlos Regalado-Gonzales, Departamento de Investigación y Posgrado, Facultad de Química, Universidad Autónoma de Querétaro (Mexico)

Prof. Mahdi Kadivar, Department of Food Science, College of Agriculture, Isfahan University of Technology (Iran)

Selected publications:

  • A. Al-Asmar, C.V.L. Giosafatto, M. Sabbah, A. Sanchez, R. Villalonga Santana, L.Mariniello. Effect of Mesoporous Silica Nanoparticles on The Physicochemical Properties of Pectin Packaging Material for Strawberry Wrapping. Nanomaterials (2020), 24;10(1).
  • M. Sabbah, M. Altamimi, P. Di Pierro, C. Schiraldi, M.  Cammarota, R.  Porta, Black edible films from protein-containing defatted cake of Nigella sativa seeds. International Journal of Molecular Sciences (2020), 21(3), 832
  • A. Al-Asmar, C.V.L. Giosafatto, L. Panzella, L. Mariniello. The Effect of Transglutaminase to Improve the Quality of Either Traditional or Pectin-Coated Falafel (Fried Middle Eastern Food). Coatings (2019), 9(5), 331.
  • Abdalrazeq M, Giosafatto C.V.L. Esposito E., FendericoM. Di Pierro P.  Porta R.  Glycerol-Plasticized Films Obtained from Whey Proteins Denatured at Alkaline pH Plasticizing effects of polyamines in protein-based films. Coatings (2019) 9(5), 322
  • M. Sabbah, P. Di Pierro, E. Dell'Olmo, A. Arciello R. Porta. Improved shelf-life of Nabulsi cheese wrapped with hydrocolloid films Food Hydrocolloids (2019) 96, 29.

Ongoing granted projects

  • Italian Ministero dell'Istruzione, dell'Università e della Ricerca : PRIN: Progetti di Ricerca di Interesse Nazionale –Bando 2017– "CARDoon valorisation by InteGrAted biorefiNery (CARDIGAN)"
  • Ministero degli Affari Esteri e della cooperazione Internazionale (2018):-Progetto di mobilità tra l'Italia e gli Stati Uniti del  Messico "Film edibili nano-rinforzati per la preparazione di nuovi materiali per il confezionamento alimentare"