AOCL- Analytical And Organic Chemistry for Life

Dr Daniele Naviglio
Room 2N-01; phone +39-081674063; email

Dr Anna Andolfi

Room 2N-22; phone +39-0812539179; email

orcid, webdocenti



Other Group members:

post-Doc: Maria Michela Salvatore


Extraction, isolation and structural identification of bioactive compounds from vegetables

Bioactive secondary metabolites produced by fungi


Extraction, isolation and structural identification of bioactive compounds from vegetables

Our main activities are the development, optimization and validation of innovative analytical methods aimed at the extraction, isolation, identification and quantification of natural compounds essentially from plants complex matrices. These activities, carried out in collaboration with research institutions and companies, have as primary aim the development of new formulations in the food and pharmaceutical sector. The research activity foresees: i) the optimization of both conventional solid-liquid extraction techniques such as maceration and percolation and of innovative technologies such as rapid dynamic solid-liquid extraction carried out using the Naviglio Extractor; ii) the isolation of the compounds of interest through the use of chromatographic techniques; iii) the structural identification of the main compounds through the use of spectroscopic (essentially NMR) and spectrometric (MS) methods; iv) the qualitative-quantitative analysis of the compounds of interest by using instrumental techniques such as GC-FID, HPLC-DAD, GC-MS and LC-MS.


Main collaboration:

Prof. Farid CHEMAT Avignon Université UMR 408, INRA - AU, GREEN UFR-ip Sciences, Campus Jean-Henri Fabre301 rue Baruch de Spinoza, BP 2123984916 Avignon Cedex 9 – France

Prof.ssa Michelina Catauro, Dipartimento di Ingegnaria, Università degli Studi della Campania Luigi Vanvitelli, via Roma 29 - 81031Aversa (CE).

Prof. Carmine Guarino, Dipartimento di Scienze e Tecnologie (DST), Università degli Studi del Sannio, via Francesco de Sanctis - 82100 Benevento BN - Italy

Prof.ssa Rosaria Sciarrillo, Dipartimento di Scienze e Tecnologie (DST), Università degli Studi del Sannio, via Francesco de Sanctis - 82100 Benevento BN - Italy

Dott. Monica Gallo Department of Molecular Medicine and Medical Biotechnology University of Naples Federico II Via Pansini, 5, 80131 Naples, Italy

Dott. Andrea Formato Department of Agricultural Science, University of Naples Federico II, via Università 100, 80045 Portici (NA), Italy


Bioactive secondary metabolites produced by fungi

Fungi can be thought as biosynthetic factories detaining the recipe for a plethora of biologically active and structurally diverse natural products (secondary metabolites) to be used as a source of original structural information.  In this respect, our group is involved in the isolation, structural and stereostructural characterization of bioactive metabolites produced in vitro essentially by fungi from diverse habitat and/or associated to organisms. To achieve these goals, the most advanced analytical and structure elucidation techniques are employed, in addition to the classic procedures of isolating and identifying bioactive fungal compounds.

The study the fungal metabolomic profiles is related to the important biological roles of secondary metabolites as virulence factors, chemical defense agents and chemical signal for communication. Furthermore, the importance to exploit natural resources is related to their potential applications as chemotherapeutic agents, pesticides, antimicrobials were evaluated in collaboration with plant pathologists, physiologists, geneticists, microbiologists, toxicologists, entomologists. Moreover, skills have been developed on the qualitative and quantitative analysis of bioactive metabolites and on their derivatization for biological activity structure correlation studies.

Main collaboration:

Prof. Eckhard Thines Institute of Biotechnology and Drug Research (IBWF), Kaiserslautern, Germania

Prof. Artur Jorge da Costa Alves e della dott.ssa Ana Cristina Estevas del Dipartimento di Biologia, CESAM (Centre for Environmental and Marine Studies) Università di Aveiro, Portogallo.

Dr. Muhammad Akbar, Department of Botany, University of Gujrat, Pakistan

Dott. Rosario Nicoletti Council for Agricultural Research and Agricultural Economy Analysis (CREA) Italia

Dott. Francesco Vinale dell'Istituto di Protezione Sostenibile delle Piante, CNR/Dipartimento di Medicina Veterinaria e Produzioni Animali, Università degli Studi di Napoli Federico II

Prof. Marco Guida e Prof. Emilia Galdiero Dipartimento di Biologia, Università degli Studi di Napoli Federico II