• CFS (CHIMICA FISICA SPERIMENTALE)

Research:

Characterization of biological model membranes and their interactions with peptides and proteins

Characterization of nanosystems: nanoparticles and nanostructured materials.

Production and characterization of biological nanovehicles

Chemistry for cultural heritage

Formulation and characterization of colloidal systems

Structural and thermodynamic characterization of biological macromolecules in solution

Structural characterization of biological macromolecules and their complexes with ligands and drugs

Characterization of biological model membranes and their interactions with peptides and proteins

Characterization of biological model membranes and their interactions with peptides and proteins: biological model membranes are produced (for example with the thin layer method) and characterized by means of spectroscopic techniques, including fluorescence spectroscopy and electron paramagnetic spectroscopy (with the spin label approach), as well as calorimetric and neutron scattering ones (neutron reflectivity), in terms of structural, dynamic, and functional properties. Their interaction with peptides and proteins is also investigated from a thermodynamic point of view through the same combination of spectroscopic and calorimetric methods.

 

Selected publications:

  • 1. R. Oliva, M. Chino, K. Pane, V. Pistorio, A. De Santis, E. Pizzo, G. D'Errico, V. Pavone, A. Lombardi, P. Del Vecchio, E. Notomista, F. Nastri, L. Petraccone "Exploring the role of unnatural amino acids in antimicrobial peptides" Sci Rep. (2018), 8(1), 8888.
  • 2. L. Lombardi, M. I. Stellato, R. Oliva, A. Falanga, M. Galdiero, L. Petraccone, G. D'Errico, A. De Santis, S. Galdiero, P. Del Vecchio. "Antimicrobial peptides at work: interaction of myxinidin and its mutant WMR with lipid bilayers mimicking the P. aeruginosa and E. coli membranes". Sci.Rep., (2017), 7, 44425.
  • 3. A. De Santis, S. La Manna, I. Russo Krauss, A.M. Malfitano, E. Novellino, L. Federici, A. Di Matteo, G. D'Errico, D. Marasco, "Nucleophosmin-1 aggregating regions associated with Acute Myeloid Leukemia interact differently with lipid membranes" BBA general subjects (2018) 1862, 967-978
  • 4. R. Oliva, P. Del Vecchio, M. I. Stellato, A. M. D'Ursi, G. D'Errico, L. Paduano, L. Petraccone. "A thermodynamic signature of lipid segregation in biomembranes induced by a short peptide derived from glycoprotein gp36 of feline immunodeficiency virus". Biochim Biophys Acta - Biomembranes, (2015), 1848, 510.
  • 5. G. Vitiello, A. Falanga , A. A. Petruk, A. Merlino, G. Fragneto, L. Paduano, S. Galdiero, G. D'Errico "Fusion of raft-like lipid bilayers operated by a membranotropic domain of the HSV-type i glycoprotein gH occurs through a cholesterol-dependent mechanism" Soft Matter (2015) 11, 3003-3016

Characterization of nanosystems: nanoparticles and nanostructured materials.

Characterization of nanosystems: nanoparticles and nanostructured materials: synthesis of nanoparticles (metal and metal oxide NPs, surfactant-based NPs) and their characterization by means of spectroscopic (UV-Vis, IR, Fluorescence, Raman spectroscopy) and light and neutron scattering techniques. Characterization of different nanostructured materials by means of IR and Raman microscopy.

 

Selected publications:

  • 1. P. Pernice, L. Sirleto, A. Vergara,, A. Aronne, M. Gagliardi, E. Fanelli, G. Righini, "Large Raman gain in a stable nanocomposite based on niobiosilicate glass", J. Phys. Chem. C., (2011), 115, 17314–17319.
  • 2. L. Sirleto, A. Vergara, A. Ferrara „Advances in Stimulated Raman scattering in nanostructures" Advances in Optics and Photonics (2017) 9, 169-217.
  • 3. A. Luchini, C. Irace, R. Santamaria, D. Montesarchio, R.K. Heenan, N. Szekely, A. Flori, L. Menichetti, L. Paduano, "Phosphocholine-decorated superparamagnetic iron oxide nanoparticles: Defining the structure and probing In vivo applications" Nanoscale (2016), 8, 10078-10086
  • 4. A. Luchini, G. Vitiello, F. Rossi, O.R. De Ballesteros, A. Radulescu, G. D‘Errico, D. Montesarchio, C.S.J. De Fernandez, L. Paduano, "Developing functionalized Fe3O4-Au nanoparticles: A physico-chemical insight" PCCP (2015), 17, 6087-6097
  • 5. A. Luchini, Y. Gerelli, G. Fragneto, T. Nylander, G.K. Palsson, M.S. Appavou, L. Paduano "Neutron Reflectometry reveals the interaction between functionalized SPIONs and the surface of lipid bilayers" Colloids and Surfaces B, (2017) 151, 76-87

Production and characterization of biological nanovehicles

Production and characterization of biological nanovehicles: Production of biological nanovehicles by encapsulation of drugs into auto-assembling proteins and/or lipids and their characterization by means of spectroscopic techniques (UV-Vis, Fluorescence, CD, EPR), light scattering technique (DLS, ELS) and X-ray crystallography.

 

Selected publications:

  • 1. N. Pontillo, F. Pane, L. Messori, A. Amoresano, A. Merlino. "Cisplatin Encapsulation within the Ferritin Nanocage: A High-Resolution Crystallographic Study". Chem Commun (Camb) (2016), 52(22):4136-9.
  • 2. G. Ferraro, D.M. Monti, A. Amoresano, N. Pontillo, G. Petruk, F. Pane, M.A. Cinellu, A. Merlino "Gold-based Drug Encapsulation within the Ferritin Nanocage: X-ray Structure and Biological Evaluation as Potential Anticancer Agent of the Auoxo3-loaded protein". Chem Commun (Camb) (2016), 52(61):9518-21
  • 3. L. Simeone, G. Mangiapia, G. Vitiello, C. Irace, A. Colonna, O. Ortona, D. Montesarchio, L. Paduano "Cholesterol-based nucleolipid-ruthenium complex stabilized by lipid aggregates for antineoplastic therapy" Bioconjugate Chem., (2012) 23, 758–770
  • 4. C. Riccardi, D. Musumeci, A. Capuozzo, C. Irace, S. King, I. Russo Krauss, L. Paduano, D. Montesarchio, ""Dressing up" an old drug: aminoacyllipids as privileged scaffolds for the functionalization of Ru(III)-based anticancer agents" ACS Biomaterials Science & Engineering (2018) 4, 163-174
  • 5. M. Monti, G. Ferraro, G. Petruk, L. Maiore, F. Pane, A. Amoresano, M.A. Cinellu, A. Merlino "Ferritin nanocages loaded with gold ions induce oxidative stress and apoptosis in MCF-7 human breast cancer cells" Dalton Transaction, (2017), 46, 15354-15362

Chemistry for cultural heritage

Chemistry for cultural heritage Archeometric research activity focuses on the developement of physico-chemical protocols on works of art for addressing hystorical and technolgical questions, in collaborations with archaeologists and Museums.

 

Selected publications:

  • 1. M. Mercurio, F. Izzo, M. Rossi, P. Cappelletti, C. Germinario, M.R. Ghiara, C. Grifa, M. Petrelli, A. Vergara, A. Langella „The characterization of natural gemstones using non-invasive FT-IR spectroscopy: new data on tourmalines" Talanta (2018) 178, 147-159.
  • 2. L. Birolo, A. Tomeo, M. Trifuoggi, F. Auriemma, L. Paduano, A. Amoresano, R. Vinciguerra, C. De Rosa, L. Ferrara, A. Giarra, A. Luchini, C. De Maio, G. Greco, A. Vergara* "A hypothesis on different technological solutions for outdoor and indoor Roman wall paintings" Archaeol. Anthropol. Sci. (2017), 9 (4), 591-602. DOI: 10.1007/s12520-016-0408-y
  • 3. R. Vinciguerra, E. Galano, F. Vallone, G. Greco, A. Vergara, I. Bonaduce, G. Marino, P. Pucci, A. Amoresano, L. Birolo, "A deglycosylation step to improve the identification of egg proteins in art samples" Analytical Chemistry (2015) 87 (20), 10178-10182.
  • 4. M. Gelzo, M. Grimaldi, A. Vergara, V. Severino, A. Chambery, A. Dello Russo, C. Piccioli, G. Corso, P. Arcari "Comparison of binder composition in Pompeian wall painting styles from Insula Occidentalis" Chemistry Central J. (2014) 8:65.
  • 5. A. Vergara, L. Mazzarella, "Microscopia Confocale Raman" in "La diagnostica nei beni culturali. Moderni metodi di indagine", a cura di L. Paolillo e I. Giudicianni, Ed. Loghia Publishing & Research, Napoli (2009), Sez. 1, Cap. 3, pp. 33-46. ISBN 978-88-95122-17-5. In italiano.

Formulation and characterization of colloidal systems

Formulation and characterization of colloidal systems: formulation and structural and functional characterization of colloidal systems, mainly based on surfactants and polymers, in the presence of salts, cosolvents and additives, for industrial applications, by means of light and neutron scattering techniques, spectroscopic techniques, polariscopy and microscopy.

 

Selected publications:

  • 1. I. Russo Krauss, R, Imperatore, A. De Santis, A. Luchini, L. Paduano, G. D'Errico, "Structure and dynamics of cetyltrimethylammonium chloride-sodium dodecylsulfate (CTAC-SDS) catanionic vesicles: High-value nano-vehicles from low-cost surfactants" Journal of Colloid and Interface Science (2017) 501, 112-122
  • 2. M. Perfetti, I. Russo Krauss, I., A. Radulescu, N. Ruocco, G. D'Errico, G.O., Bianchetti, L. Paduano, "Poly-ethylene-vinyl alcohol microgels prepared through salting out: rationalizing the aggregation process and tuning the microstructural properties" Polymer (2018) 137, 122-131
  • 3. G. Vitiello, G. Mangiapia, E. Romano, M. Lavorgna, S. Guido, V. Guida, L. Paduano, G. D'Errico "Phase behavior of the ternary aqueous mixtures of two polydisperse ethoxylated nonionic surfactants" Colloids and Surfaces A, (2014) 442, 16-24
  • 4. N. Ruocco, H. Frielinghaus, G. Vitiello, G. D'Errico, L.G. Leal, D. Richter, O. Ortona, L. Paduano "How hydrophobically modified chitosans are stabilized by biocompatible lipid aggregates" Journal of Colloid and Interface Science (2015) 452, 160-168
  • 5. G. D'Errico, L. Paduano, O. Ortona, G. Mangiapia, L. Coppola, F. Lo Celso, "Temperature and concentration effects on supramolecular aggregation and phase behavior for poly(propylene oxide)-b-poly(ethylene oxide)-b-poly(propylene oxide) copolymers of different concentration in aqueous mixtures. 2" Journal of Colloid and Interface Science (2011), 359, 179-188

Structural and thermodynamic characterization of biological macromolecules in solution

Structural and thermodynamic characterization of biological macromolecules in solution: characterization of biological macromolecules, proteins and nucleic acids, by analysis of: a) structure and stability via spectroscopic (CD, UV-visible and fluorescence), electrophoretic and calorimetric (DSC, ITC) techniques. Thermodynamic characterization of biological macromolecules in solution and development of thermodynamic models for interpretation of experimental data of thermal and chemical denaturation of proteins and nucleic acids.

 

Selected publications:

  • 1) M. Vigorita, S. Cozzolino, R. Oliva, G. Graziano, P. Del Vecchio "Counteraction ability of TMAO toward different denaturing agents" Biopolymers (2018) e23104. DOI: 10.1002/bip.23104.
  • 2) I. Russo Krauss, V. Napolitano, L. Petraccone, R. Troisi, V. Spiridonova, C.A. Mattia, F. Sica "Duplex/quadruplex oligonucleotides: role of the duplex domain in the stabilization of a new generation of highly effective anti-thrombin aptamers" International Journal of Biological Macromolecules (2018) 107, 1697-1705
  • 3) R. Berisio, F. Squeglia, A. Ruggiero, L. Petraccone, M.I. Stellato, P. Del Vecchio "Differential thermodynamic behaviours of the extra-cellular regions of two Ser/Thr PrkC kinases revealed by calorimetric studies"., Biochim Biophys Acta. -Proteins and Proteomics, (2015), 1854, 402.
  • 4) Iolanda Fotticchia, Concetta Giancola, Luigi Petraccone. "G-quadruplex unfolding in higher-order DNA structures" Chem Commun., (2013), 49, 9488
  • 5) A. Merlino, I. Russo Krauss, I. Castellano, M.R. Ruocco, A. Capasso, E. De Vendittis, B. Rossi, F. Sica "Structure and flexibility in cold-adapted iron superoxide dismutases: the case of the enzyme isolated from Pseudoalteromonas haloplanktis" Journal of Structural Biology (2010) 172, 343-352

Structural characterization of biological macromolecules and their complexes with ligands and drugs

Structural characterization of biological macromolecules and their complexes with ligands and drugs: structural characterization by means of X-ray crystallography of biological macromolecules (nucleic acids and proteins) free and in complex with ligand and drug (metal-complexes, small molecules, other biomacromolecules, etc.). Support of spectroscopic techniques and Raman micro-spectroscopy to investigate binding, stability of the complexes and induced structural changes.

 

Selected publications:

  • 1. I. Russo Krauss, A. Merlino, A. Randazzo, E. Novellino, L. Mazzarella, F. Sica "High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity" Nucleic Acids Research (2012) 40, 8119-8128
  • 2. L. Messori and A. Merlino. "Cisplatin binding to proteins: a structural perspective". Coordination Chemistry Reviews (2016), 315, 67-89.
  • 3. I. Russo Krauss, V. Spiridonova, A. Pica, V. Napolitano, F. Sica "Different duplex/quadruplex junctions determine the properties of anti-thrombin aptamers with mixed folding" Nucleic Acids Research (2016), 44, 983-991
  • 4. A. Merlino. "Interactions between proteins and Ru compounds of medicinal interest: a structural perspective". Coordination Chemistry Reviews (2016), 326, 111–134.
  • 5. A. Pica, I. Russo Krauss, V. Parente, H. Tateishi-Karimata, S. Nagatoishi, K. Tsumoto, N. Sugimoto, F. Sica "Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers" Nucleic Acid Research (2017) 45, 461-469